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. 2017 Sep 12;12(9):e0184499. doi: 10.1371/journal.pone.0184499

Fig 5. Immunofluorescence analysis of Msln splicing variant distribution in transfected COS7 cells.

Fig 5

COS7 cells transfected with plasmid DNAs encoding Msln splicing variants A, H, S, U, W, and Y (red) were transduced to express plasma membrane-bound GFP (green), and analyzed by microscopy. While recombinant Msln A and W variants are associated with the plasma membrane (white arrowheads) and cytoplasm compartments, the remaining Msln are observed predominantly in the cytoplasmic/perinuclear area of the cells (purple). Nuclear stain is DAPI (blue). Magnification 630X.