Table 1. Sequences of PCR primer sets used for gene expression analysis and cloning.

Identifier	Sequence	Experiment
Rat Msln forward	GTGGTGTGAGTTGAGGGGTG	RT-PCR, Sequencing
Rat Msln reverse	GGGATGCTGTGGACAATGGA	RT-PCR, Sequencing
Rat Msln UTR forward	TGTGTCCAAACAGTGGTGTG	RT-PCR, Sequencing, Cloning
Rat Msln UTR reverse	CAGGAGCCTTAGGAGTGGTG	RT-PCR, Sequencing, Cloning
Mouse Msln forward	TGTCTCCAAACAGTGGTGTG	RT-PCR, Sequencing
Mouse Msln reverse	CAGTAGAGCTGGGACCAGGA	RT-PCR, Sequencing
Rat/Mouse Gapdh forward	TTGTGCAGTGCCAGCCTC	RT-PCR
Rat/Mouse Gapdh forward	CTGGAAGATGGTGATGGGCT	RT-PCR
Rat Msln OEP forward 1	TCTAGAATGGCCTTGCCAACAGCCCAACCCTACCCATACGATGTTCCAGATTACGCT	Overlap extension PCR
Rat Msln OEP forward 2	TACCCATACGATGTTCCAGATTACGCTCTGCTGGGGTCCTGTGGAAGC	Overlap extension PCR
Rat Msln OEP reverse	ACCGGTTCAGCTCAGTCTTAAAGCT	Overlap extension PCR
IVT forward	GCGAATTAATACGACTCACTATAGGGCTTAAGTATAAGGAGGAAAAAATATGGCCTTGCCAACAGCCCAACCCTAC	In vitro translation PCR
IVT reverse	AAACCCCTCCGTTTAGAGAGGGGTTATGCTAGTCAGCTCAGTCTTAAAGCTGAGAG	In vitro translation PCR
Gibson rat Msln forward	CCGTTTAAACTCATTACTAACCGGTTCAGCTCAGTCTTAAAGCTGAGAGC	Cloning
Gibson rat Msln reverse	ACCGATCCAGCCTCCGGACTCTAGAATGGCCTTGCCAACAGCC	Cloning
M13 forward	GTAAAACGACGGCCAG	PCR, Sequencing
M13 reverse	CAGGAAACAGCTATGAC	PCR, Sequencing
CMV forward	CGCAAATGGGCGGTAGGCGTG	PCR, Sequencing
TKpA reverse	CTTCCGTGTTTCAGTTAGC	PCR, Sequencing

CMV, cytomegalovirus; IVT, in vitro translation; OEP, overlap extension PCR; TKpA, thymidine kinase polyadenylation signal; UTR, untranslated region.