Fig 8. PilB and FimX interactions are seen in a split luciferase complementation assay.
PA103 co-expressing Nluc- and Cluc-fusion protein constructs as indicated (or empty vector “V”) were grown overnight on LB plates containing appropriate antibiotics and 0.05% arabinose. Bacteria were harvested by scraping, resuspended in PBS and normalized by OD600. RLU (luciferase units/OD600) was measured on addition of 1 μM coelenterazine substrate to the bacterial cells. Bars represent the mean ± SD of two technical replicates from a representative experiment. Values for FimX-FimX are set to 100%.