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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Hum Immunol. 2017 Mar 4;78(4):342–349. doi: 10.1016/j.humimm.2017.03.002

Figure 2.

Figure 2

(A). The ddPCR reactions were carried out with one isolated genomic DNA containing DRB1*03 at a fixed concentration shown in green, spiked with another isolated genomic DNA sample containing DRB1*04 shown in blue that are serially diluted. The copy number of targeted alleles was measured. Scatterplots of three representing assays (1, 3, 5) with corresponding probes (HEX 03 and FAM 04) showing the segregating droplets. (B). The sensitivity of ddPCR was tested using genomic DNA (HLA-DRB1*11/11). The copy number of targeted allele (DR11) was measured at each sample with indicated concentration.