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. 2017 Sep 12;6:e27480. doi: 10.7554/eLife.27480

Figure 5. TAILS analysis identified new rhoptry proteins as ASP3 substrates.

(A) Schematic representation of the different candidate identified during the TAILS analysis. SP – signal peptide, TM – transmembrane, SAP - SAF-A/B, Acinus and PIAS domain, TSP1 - thrombospondin-1, S-TKc – Serine-Threonine kinase catalytic domain. (B), (D), (F), (H), (J) TAILS 3, 5, 6 7 and 8 were localized to the rhoptries and (C), (E), (G), (I) (K) showed impaired processing upon ASP3 depletion. Arrowheads represent pro and mature forms of the proteins. MIC6 was used as control. (L, M) Altered localization of TAILS3 and TAILS8 in the rhoptries upon ASP3 depletion.

Figure 5.

Figure 5—figure supplement 1. Expression profile of TAILS candidates.

Figure 5—figure supplement 1.

Expression profile of the TAILS1-8 as well as RON2, ROP7 and MIC2 as representative examples of rhoptry neck, rhoptry bulb and microneme proteins, respectively.
Figure 5—figure supplement 2. Effect of ASP3 knockdown on the localization of the TAILS candidates.

Figure 5—figure supplement 2.

(A–C) Conditional depletion of ASP3 did not affect the localization of the TAILS5, TAILS6 and TAILS7 in the rhoptries.