Chart 1.
Stainings used in cases of chromoblastomycosis and their purposes in the course of treatment with itraconazole
| STAINING | PURPOSE | |||
|---|---|---|---|---|
| Hematoxylin-eosin | Analysis of tissue modifications | Definition of the degree of tissue changes | ||
| Epidermis | Morphology | E1: | Atrophy: decreased epidermal thickness with loss of papillary pattern | |
| E2: | Hyperplasia: increased number of epidermal cells | |||
| E3: | Pseudoepitheliomatous hyperplasia: increased cell numbers, thickening of the epidermis, rare mitoses without loss of polarity and elongation of the epithelial cones with epidermal shoots within the dermis | |||
| Dermis | Inflammatory infiltrate | D1: | Local lymphoplasmo-histiocytic | |
| D2: | Lymphoplasmo-histiocytic and mononuclear with sparse granulomas | |||
| D3: | Lymphoplasmo-histiocytic, polymorphonuclear and mononuclear with various granulomas and microabscesses | |||
| Hipodermis | Tissue fibrosis | F1: | Predominantly in hypodermis | |
| F2: | Predominantly in hypodermis and reticular dermis | |||
| F3: | In hypodermis, reticular and papillary dermis | |||
| Fontana- Masson | Parasite analysis in host tissue | |||
| Distribution of fungi in the epidermis, dermis and hypodermis and visualization of their aspects | ||||