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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Genes Brain Behav. 2017 May 2;16(7):699–708. doi: 10.1111/gbb.12384

Figure 3. Midkine knockout mice consume more ethanol than controls in the drinking in the dark (DID) test.

Figure 3

Male (a, c) and female (b, d) wild type (Mdk +/+, filled circles) and homozygous midkine knockout (Mdk −/−, open squares) mice underwent a modified DID test, in which they were provided 20% ethanol in water (v/v) for 4 h per day, 4 days per week for 4 weeks. (a, b) Ethanol consumed in g ethanol/kg body weight/4 h during the first week of the DID test. There were significant effects of genotype for males and females and a significant effect of day for females. (c, d) Average ethanol consumed each week, expressed as g ethanol/kg body weight/4 h. There was a significant effect of genotype in males. Male Mdk +/+, n = 9; male Mdk −/−, n = 9; female Mdk +/+, n = 7; female Mdk −/−, n = 7. * P < 0.05 by two-way ANOVA. Data are presented as the mean ± SEM.