Antibodies |
Mouse monoclonal antibody anti-β2/3 subunit of GABAA receptor; clone BD-17 |
Millipore |
Cat#MAB341; RRID: AB_2109419 |
Anti-G-protein Goα; clone 2A |
Millipore |
Cat#MAB3073; RRID: AB_94671 |
Anti-PKCα, mc5 |
Sigma-Aldrich |
Cat#P4334; RRID: AB_477345 |
Alexa Fluor®488 Donkey anti-mouse IgG antibody |
Invitrogen |
Cat#A21202; RRID: AB_141607 |
Alexa Fluor®647 Donkey anti-mouse IgG antibody |
Invitrogen |
Cat#A31571; RRID: AB_162542 |
Mouse IgG F(ab)2 monoclonal antibody |
Jackson Immunoresearch |
CAT#115-006-006 |
Biotin-SP AffinityPure Donkey anti-mouse IgG |
Jackson Immunoresearch |
Cat#715-065-150; RRID: AB_2307438 |
Chemicals, Peptides, and Recombinant Proteins |
SCH23390; dopamine D1 receptor antagonist |
Tocris |
Cat#0925 |
Spiperone, dopamine D2-like receptor antagonist |
Sigma-Aldrich |
Cat#S7395 |
Gabazine, GABAA receptor antagonist |
Tocris |
Cat#1262 |
TPMPA, GABAC receptor antagonist |
Tocris |
Cat#1040 |
Alexa Fluor 488 Hydrazide |
Invitrogen |
Cat#A10436 |
Lectin Peanut Agglutinin (PNA) from Arachis Hypogaea; Alexa Fluor ® conjugate |
Invitrogen |
Cat#L21409
|
Osmium tetroxide |
Fisher Scientific |
Cat#AC19118-0010 |
Gold Chloride Trihydrate |
Fisher Scientific; MP Biomedicals |
Cat#0215252601 |
Silver Nitrate |
Fisher Scientific |
Cat#BP2546100 |
Critical Commercial Assays |
Vectastain Standard Elite ABC Kit |
Vector Labs |
Cat#3333333 |
DAB Substrate Kit |
Vector Labs |
Cat#SK-4100 |
Epon812 Embedding kit |
Electron Microscopy Science |
Cat#50-980-391 |
Experimental Models: Organisms/Strains |
Dutch-belted rabbits |
Myrtle’s; Covance |
N/A |
Software and Algorithms |
VisionWorks for Electrophysiology |
Vision Research Graphics, Durham, NH |
http://www.vrg.com |
Clampfit 10.1 |
Molecular Devices |
https://www.moleculardevices.com/systems/conventional-patch-clamp/pclamp-10-software |
R |
R Foundation for Statistical Computing, Vienna, Austria |
https://www.r-project.org/about.html |
GraphPad Prism, Version 6.01 |
GraphPad Software, Inc. |
https://www.graphpad.com |
ZEN Software |
Carl Zeiss, Germany |
https://www.zeiss.com/microscopy/us/products/microscope-software/zen.html |
JACoP plugin for ImageJ |
NIH |
https://imagej.nih.gov/ij/plugins/track/jacop.html |