Perilipin 2 localizes almost exclusively to lipid droplets and does not significantly recruit ATGL, ABHD5, or HSL to lipid droplets under either basal or lipolytically stimulated conditions. Perilipin 2 is relatively permissive to lipolysis; lipolysis under basal conditions is approximately comparable to lipolysis when PKA is activated. Perilipin 2 is not a substrate for PKA, although HSL, ATGL, and ABHD5 are substrates. The localization of ATGL with ABHD5 and HSL to perilipin 2 coated lipid droplets is likely transient under both conditions. It is unknown whether or not perilipin 2 affects MAGL localization or activity.