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. 2017 Jun 14;16(5):994–1005. doi: 10.1111/acel.12623

Figure 3.

Figure 3

Snf1/AMPK deletion suppresses the glucose starvation‐like response in ChES. (A) The level of phosphorylated Snf1 is increased in ChES. Data represent quantification (ImageJ) of Western blot results from three independent experiments. Phospho‐Snf1 band intensity was normalized to the intensity of total Snf1 and β‐actin (loading control). Control is wild‐type yeast with an empty vector. Data are mean ± SD from three independent cultures, each performed in triplicate. ***P < 0.001; **P < 0.01; *P < 0.05 (ANOVA plus post hoc). (B) Snf1 is required to trigger the glucose starvation‐like response by enhanced chaperone activity. Transcript levels of target genes in ChES in the absence of Snf1 are compared to the control (wild‐type yeast with an empty plasmid). UBC6 was used for normalization. Color of the squares on the heat map corresponds to the mean value of the log fold change from three biological and three technical replicates. (C) Oxygen consumption of the ChES declines in the absence of Snf1. In brackets are the mean values of oxygen consumption of the corresponding ChES in the wild‐type background, added for comparison. As a control strain, the ΔSnf1 yeast with an empty vector was used. Data are mean ± SD from at least three independent cultures, each performed in triplicate. ***P < 0.001; **P < 0.01; *P < 0.05 (ANOVA plus post hoc).