Skip to main content
. 2017 Jun 14;16(5):1035–1042. doi: 10.1111/acel.12631

Figure 6.

Figure 6

Suppression of p53 activity by TAZ via direct interaction. (a) Immunoblotting analysis of p53 in the testis of WT and KO mice (12 months old, n = 6/group). (b–d) 293T cells were transfected with the reporter gene either PG13‐luc (b) or p21‐luc (d) and/or TAZ expression vector. Expression of p53 and TAZ was detected by immunoblotting analysis (c). The reporter activity was determined after normalization with β‐galactosidase activity and given as the fold induction compared to mock control. (e, f) 293T cells were transfected with Flag‐tagged p53 and Myc‐tagged TAZ expression vector. Whole cell extracts (WCE) were harvested and used for immunoprecipitation with anti‐Flag antibody, followed by Immunoblot analysis (e). Or cell extracts were incubated with biotinylated p21 promoter, followed by precipitation and immunoblotting (f). The data in panel b and d indicate the mean ± SD of three independent experiments. ***P < 0.0005. Representative blots of at least three experiments are shown in panels a, c, e, and f.