Skip to main content
. 2017 Aug 11;16(5):1155–1167. doi: 10.1111/acel.12652

Figure 1.

Figure 1

Effect of age and CR on cardiomyocyte hypertrophy. (A) Representative images using FITC‐conjugated WGA staining for cardiac tissues from AL and CR mice at 6, 15, and 22 months of age. Scale bars, 20 μm. (B) Quantitation of cardiomyocyte cross‐sectional area. Mean ± SEM, n = 200 cardiomyocytes for cross‐sectional area quantification. (C) Quantitation of cardiomyocyte number. Mean ± SEM, n = 3 whole cross sections of left ventricles per group for cardiomyocyte number quantification. (D) mRNA quantification of the hypertrophic marker genes. *P < 0.05 vs. age‐matched AL group, **P < 0.01 vs. age‐matched AL group. #P < 0.05 vs. 6 months AL group, ##P < 0.01 vs. 6 months AL group. &P < 0.05 vs. 15 months AL group, &&P < 0.01 vs. 15 months AL group.