Figure 4.

Effect of stress on FOXO3 interaction with neighborhood genes. FISH was used to localize FOXO3 and the two most distant genes, HACE1 and LAMA4, in the FOXO3 neigborhood in lymphoblastoid cell lines in response to cellular stress caused by 200 μm H₂O₂ + serum deprivation. (a) Shows the order of the three genes and color of fluorescent dye. (b) Shows the measurements taken. (c) Is an example of FISH results for one of ten lymphoblastoid cell lines from different subjects. (d) Depicts a model in which neighboring genes aggregate to form a stronger transcription complex in response to stress (only the three genes indicated, of 46 in the region, are shown).