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. 2017 Jul 5;16(5):976–987. doi: 10.1111/acel.12616

Figure 4.

Figure 4

Levels of the longevity gene Sirt1, autophagy protein markers, mitophagy protein markers, mitochondrial proteins UCP‐2, PGC‐1α, and aconitase activity in myocardium from male young (3–4 months) or old (24–26 months) WT and Akt2−/− mice. (A) Representative gel blots depicting levels of Sirt1, LC3BI/II, Atg7, p62, PINK1, Parkin, BNIP3, and respective loading controls (GAPDH and COX‐IV) using specific antibodies; (B) Sirt1 levels (n = 10, 9, 9, and 8 mice for the four animal groups shown from left to right); (C) LC3B‐II‐to‐LC3B‐I ratio (n = 16, 18, 13, and 16 mice from left to right); (D) Beclin‐1 levels (n = 7, 11, 7, and 8 mice from left to right); (E) Atg7 levels (n = 9, 9, 9, and 8 mice from left to right); (F) p62 levels (n = 8, 11, 9, and 8 mice from left to right); (G) Mitochondrial PINK1 levels (normalized to COX‐IV, n = 9, 10, 12, and 10 mice from left to right); (H) Mitochondrial Parkin levels (normalized to COX‐IV, n = 9, 10, 9, and 9 mice from left to right); (I) Mitochondrial BNIP3 levels (normalized to COX‐IV, n = 11, 8, 11, and 11 mice from left to right); (J) UCP‐2 levels (n = 6, 7, 5, and 5 mice from left to right); (K) PGC‐1α levels (n = 6, 7, 5, and 5 mice from left to right); Insets: Representative gel blots depicting levels of UCP‐2, PGC‐1α, and GAPDH using specific antibodies; and (L) Aconitase activity (n = 8 mice per group). Mean ± SEM, *P < 0.05 vs. WT young group, # P < 0.05 vs. WT old group.