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. 2017 Jun 30;16(5):1062–1072. doi: 10.1111/acel.12634

Figure 3.

Figure 3

Inhibition of G9a/GLP complex ameliorates Aβ‐induced deficits in synaptic tagging/capture (STC). (A) STC was tested using ‘strong before weak’ (SBW) paradigm. Induction of early‐LTP in S2 (open circles) by WTET 60 min after the late‐LTP induction in S1 (filled circles) by STET resulted in late‐LTP in both the synaptic inputs, thereby expressing STC (n = 7). (B) The same SBW paradigm when used in the hippocampal slices pretreated with Aβ failed to express late‐LTP in both the inputs S1 and S2 (n = 6). (C, E) Inhibition of G9a/GLP complex activity by UNC 0638 (UNC, 150 nm) or BIX 01294 (BIX, 500 nm) restored the synaptic tagging and capture in Aβ‐treated slices. STET in the presence of either UNC or BIX resulted in late‐LTP in S1 (filled circles) and the early‐LTP in S2 (open circles) was transformed into late‐LTP (C, n = 8) (E, n = 8). (D,F) Application of WTET 30 min after the washout of UNC (D, 150 nm, n = 9) or BIX (F, 500 nm, n = 5) in S1 (filled circles) resulted in an early‐LTP. Control potentials in S2 (open circles) were stable during the recording period. Symbols and analog traces as in Fig. 1.