Figure 1.

OsERF48 is a nuclear protein with transcriptional activation. (a) Relative expression of OsERF48 in response to abiotic stresses. Two‐week‐old seedlings were exposed to air‐drying (drought), 400 mm NaCl (salt), 100 μm abscisic acid (ABA) at 28 °C and at 4 °C (low temperature) for the indicated time points. OsUbi1 expression was used as an internal control. Values are the means ± SD (standard deviation) of three independent experiments. (b) Subcellular localization of OsERF48 in rice protoplasts. Protoplasts were transiently co‐transformed with OsERF48‐GFP and the nuclear localization control OsNF‐YA7‐mCherry. Fluorescence was observed using a confocal microscope. (c–d) Transactivation activity of OsERF48 using a yeast system. (c) Schematic structure of OsERF48 full length and deletion mutants. (d) Transformed yeast cells harbouring the indicated constructs on SD/‐Trp and SD/‐Trp/AbA²⁰⁰/X‐α‐gal. NC, negative control (pGBKT7); PC, positive control (pGBKT7‐53 + pGADT7‐RecT); AP2/ERF, AP2/ERF domain, CMI, conserved motif of group I, GBD, GAL4 DNA‐binding domain.