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. 2017 Mar 27;15(10):1295–1308. doi: 10.1111/pbi.12716

Figure 5.

Figure 5

Chromatin immunoprecipitation (ChIP)‐qPCR and transient protoplast expression assays showing that OsERF48 interacts with the OsCML16 promoter. (a–h) Two‐week‐old ROX‐Myc Os ERF 48 and nontransgenic (NT) roots were used in the ChIP‐qPCR experiments with an anti‐myc antibody. (a–d) Promoter region showing ChIP‐qPCR target positions (P1 to P4 or P1 to P3) with AP2/ERF cis‐regulatory elements. (e–h) ChIP‐qPCR data show an enrichment of chromatin DNA fragments at the indicated promoter region compared to NT plants. P1 to P4 in the OsCML16 promoter (a, e), P1 to P3 in the OsDREB1c promoter (b, f) and OsLEA24 promoter (c, g), and P1 to P3 in the Os02g0771600 gene promoter as a negative control (d, h). The relative enrichment was normalized with total input. Values are the means ± SD of three independent experiments. (i, j) Transient protoplast expression assay using a dual‐luciferase reporter system. (i) Schematic diagram of the reporter, internal control and two effector constructs. (j) Relative fLUC (fLUC/rLUC) activity in rice protoplasts. Values are the means ±  SD of three independent experiments.