Figure 4.

Montelukast increases Nrf2 nuclear translocation. Immunofluorescent staining for Nrf2 was performed 24 h following chlorine (Cl₂) exposure. (A, panels i–iv) Nuclei were stained with Hoechst. (A, panels v–viii) Illustrative images showing airways stained for Nrf2. (A, panels ix–xii) Merge of nuclear and Nrf2 staining. (B) Quantification of Nrf2 nuclear translocation using Image ProPlus software. Nrf2 translocation occurred after chlorine exposure, and this translocation was enhanced by montelukast treatment. MC + Air, MK + Air n = 6 per group; MC + Cl₂, MK + Cl₂ n = 10 per group; 10–20 airways analysed per mouse. *P < 0.05, significantly different as indicated.