Figure 4.

Effect of chlorpromazine, ketanserin or L‐741626 on the butyrate‐induced activation of ERK1/2 in DRG neurons. Rats were intracolonically administered sodium butyrate or saline twice daily for 3 days (A–D). On the next day after the last administration, chlorpromazine (4 mg·kg⁻¹) or vehicle (saline) was administered p.o. to these rats (A, B). Rats treated with butyrate were given ketanserin (5 mg·kg⁻¹), L‐741626 (10 mg·kg⁻¹) or vehicle (1% methylcellulose) (C, D), i.p. Two hours (A, B) or 15 min (C, D) later, the L5‐S1 DRG were removed from rats and tissues were subjected to Western blot analysis. (A) and (C) show representative data of immunoblotting. (B) and (D) show the relative data of band intensity at (A) and (C) respectively. Values are mean ± SEM; ^* P < 0.05, vs. vehicle.