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. 2017 Sep 12;7:11271. doi: 10.1038/s41598-017-11249-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of EVs. T-lymphoblasts and melanoma EVs, isolated by SEC, were analysed by bead-based flow cytometry using antibodies against common EV markers. Antibodies used were anti-CD59 VJ1/12, anti-CD63 TEA3/10, anti-CD81 5A6, anti-CD9 VJ1/20, and anti-MHC-I W6/32 mAbs. Data correspond to the mean ± SEM of the MFI fold change referred to the negative control, in 3–5 independent experiments.