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. 2017 Sep 12;7:11354. doi: 10.1038/s41598-017-09902-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of STED power on de-excitation, bleaching and fluorescence induced by the STED beam. (a–c) De-excitation, bleaching and STED-light-induced fluorescence vs. time-averaged power for Gaussian STED pulses of 130 fs (a), 200 ps (b) duration, and for CW STED (c). The hollow circles in (c) indicate CW STED operation with time-gating of detected fluorescence (see text). Measurements in (a–c): ATTO647N. (d) STED-light-induced fluorescence vs. power for different dyes and different pulse durations. (e) Bleaching vs. power for different dyes and different pulse durations. Note the log-log representation of data in (d,e).