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. 2017 Sep 12;7:11354. doi: 10.1038/s41598-017-09902-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Limitations of the photobleaching model in explaining the data at high STED powers (ATTO647N) (see text). (a,b) Comparison between experimental and modelled data for de-excitation, bleaching and STED-light-induced fluorescence vs. STED power. (a) represents a subset of the data shown in (b). Circles and squares correspond to two independent measurement series). (c) Extended model of states possibly involved in photobleaching³², including an additional excited singlet state S_n, and two triplet states T₁ and T_n. ISC: intersystem crossing; IC: internal conversion; ESA: excited-state absorption processes.