Skip to main content
. 2017 Sep 12;7:11306. doi: 10.1038/s41598-017-11751-7

Figure 2.

Figure 2

AZD4547 impairs stem cell populations alongside inhibition of RTK signaling in vitro. (a) BT474 and SKBR3 cells were treated with AZD4547 (0 or 3 µM) for 16 hours. Then, ALDH activity was measured using an ALDEFLUOR assay to determine the percentage of ALDH+ cells. Representative flow cytometry plots are presented in the panels to the left. (b) MDA-MB-361 and BT474 cells were treated with AZD4547 (0, 3, or 5 µM) for 7 days. Primary tumorspheres were counted and imaged before harvesting. A single cell suspension of primary tumorsphere cells were replated for 7 days to allow secondary sphere formation. The tumorsphere efficiency (number of tumorspheres formed/2000 cells plated) was recorded. Representative images of primary spheres are shown in the panels below the graphs. (c) Western blots (cropped) are shown for the indicated proteins in MDA-MB-361, BT474, and SKBR3 cells treated with AZD4547 (0, 1, 3, or 5 µM) for 72 hours. All values are presented as the mean ± S.E. (* P ≤ 0.05, ** P ≤ 0.01).