Fig. 1.

GCN5L1 liver knockout attenuated liver mitochondrial protein acetylation. a Schematic of the GCN5L1 gene showing exon 3 flanked by two loxP sites and the subsequent recombination with mice harboring the albumin-Cre-Recombinase to facilitate excision of exon 3. b Immunoblot showing GCN5L1 expression in heart, brain, kidney, and liver of wild type (WT) and GCN5L1 LKO mouse tissue lysates. c Immunoblot showing GCN5L1 levels in whole liver and isolated mitochondrial lysates of WT and LKO mice. d Immunoblot analysis of mitochondrial protein acetylation and cytosolic protein acetylation in GCN5L1 LKO mice and control (flox/flox) littermates. 30 μg of isolated mitochondrial protein and 60 μg of cytosolic protein were loaded for immunoblot analysis (n = 4 independent experiments)