Fig. 3.

GCN5L1 LKO downregulated cytosolic gluconeogenic enzymes. a The transcript levels of the rate-controlling enzymes of glycogenesis and glycogenolysis in control and LKO primary hepatocytes (n = 5 paired sets of primary hepatocytes). b, c Mitochondrial gluconeogenic enzyme pyruvate carboxylase(PC) RNA (b) and protein (c) levels in primary hepatocytes (n = 5 paired sets of primary hepatocytes). d RNA levels of cytosolic rate controlling enzymes G6Pase and PEPCK in primary hepatocytes (n = 6 paired sets of primary hepatocytes). e Primary hepatocytes were isolated and stimulated with or without glucagon (100 nM) for 6 h followed by the assessment of the levels of G6Pase and PEPCK by immunoblot analysis. Values are expressed as mean ± s.e.m. **P < 0.01 versus respective control groups by Student’s t-test. Glycogen synthase 2 (Gys2), UDP-glucose pyrophosphorylase 2 (Ugp2), Glucokinse (GCK) and Glycogen phosphorylase L (Pygl)