Fig. 4.

GCN5L1 LKO impaired gluconeogenesis. a ​Glucose production was measured in the culture medium of primary hepatocytes isolated from GCN5L1 LKO and littermate controls at 5 h after treatment with or without 100 nM glucagon. ​Glucose production was normalized to protein content (n = 3 independent experiments). b HeLa cells were transiently transfected with Mt-GCN5L1 plasmid, followed by immunofluorescence staining and confocal analysis. Tom20 serves as mitochondrial marker. Scale bar, 5 μm. c Primary hepatocytes were isolated and transfected with adenoviral expression of vector control, GCN5L1, or Mt-GCN5L1. RNA was analyzed at 36 h after transfection (n = 3 independent experiments). Values are expressed as mean ± s.e.m. **P < 0.01 versus respective groups by Student’s t-test