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. 2017 Sep 12;7:11340. doi: 10.1038/s41598-017-11860-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BBR inhibits Nrf2 nuclear translocation. (A) Representative IHC analysis of Nrf2 in the livers of HFD rats treated with BBR or vehicle for 16 weeks. (B) Immunofluorescence staining for Nrf2 (red) and nuclei (blue, DAPI) in Huh7 cells treated with FFA and BBR. (C) Quantification of cells positive for nuclear Nrf2. The average percentages of cells with Nrf2 in nucleus were quantified from a randomly selected pool of 3–6 fields in each group. (D) Western blots for Nrf2-enriched fractions in Huh7 cells treated with FFA and BBR. The results are expressed as the mean ± SEM. *p < 0.05 vs control, ^#p < 0.05 vs FFA.