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. 2017 Sep 12;7:11353. doi: 10.1038/s41598-017-11378-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DXM did not regulates Th1 and Th17 expansion in vitro.Th1 and Th17 cells were expanded from DBA/1 mouse CD4+ T cells in vitro in the presence of cytokines and treated with DXM. (a) The frequency of Th1 and Th17 cells were examined by flow cytometry. (b) The production of IFN-g and IL-17 cytokine in culture supernatant were determined by ELISA assay. *p < 0.05, **p < 0.01, ***p < 0.001, versus PBS treated group, as determined by a one-way ANOVA with Tukey’s multiple comparison test.