Figure 3.

Ethylene biosynthetic pathway induction by pre-conditioning treatment in cold-stored peach fruit. Changes on ACS activity (A), ACC content (B), MACC content (C), ACO activity (D) and ethylene production (E) in fruit harvested at the commercial harvest (CH) or tree ripe (TR) stages and of CH fruit that was pre-conditioned (PC). Ethylene synthesis was measured prior to cold treatment (pre-cold period), just after 40 d cold at 0 °C (0 d at 0 °C), and after three days ripening at 20 °C. Additional experimental details as described (Fig. 1). Different letters above bars indicate significant differences between treatments (P = 0.05) at each time point. Values are means ± SE (n = 6).