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. 2017 Sep 12;7:11358. doi: 10.1038/s41598-017-11933-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Metabolic differences and transcriptional regulation of valine and isoleucine biosynthesis in pre-cold and CI-symptomatic period. (A) Heat map representing relative abundance of primary metabolites analyzed prior to cold period (CH, TR, PC) and after three days ripening at 20 °C following 40 d cold treatment (CH 40 + 3, TR 40 + 3, PC 40 + 3) compared to CH. Additional experimental details were as described (Fig. 1). Normalized values are shown on a colour scale at the bottom of the figure, which is proportional to the concentration of each metabolite. Numerical data are provided (Supplementary Table S6). (B) Superpathway of valine and isoleucine biosynthesis in plants. Valine and isoleucine metabolite profiles for peach fruit prior to chilling and at the CI-symptomatic stage are shown (see also Fig. 8 and Supplementary Table S6). (C) Expression profiles for acetolactate synthase (ALS2), ketol-acid reductoisomerase (KARI) and dihydroxy-acid dehydratase (DAD) in peach fruit. Mesocarp tissue sampled immediately after harvest and before cold treatment (0), just after 40 d cold (40 + 0) and after three days ripening at 20 °C following 40 d cold treatment (40 + 3). Additional experimental details were as described (Fig. 1). Determinations were performed in triplicate. The vertical bar in each particular figure plate represents the least significant difference (LSD, P = 0.05), which was used for means comparison among treatments and time points.