Table 2.

List of primers for amplifying target genes.

Gene	Primer name	Primer sequence	Restriction enzyme
daf-16	daf-16-F	5′ AAAACTGCAGAGTACAGCAATTCCCAAATGAAA 3′	Pst I
	daf-16-R	5′ CCCAAGCTTAATTGGATTTCGAAGAAGTGGAT 3′	Hind III
skn-1	skn-1-F	5′ AAAACTGCAGGGGTACCCACTTGCCCTATT 3′	Pst I
	skn-1-R	5′CCCAAGCTTTCATTTCAGCCACTCACTGC 3′	Hind III
hsf-1	hsf-1-F	5′AAAACTGCAGGAAAAAAAGTAGGAGCAAAAAAT3′	Pst I
	hsf-1-R	5′CGGGGTACCAGTCAAAAAGCTGAAAAAATCGG3′	Kpn I
hsp16.2	hsp16.2-F	5′ CGGGGTACC ATTCAGCAGATTTCTCTTCGACGATT 3′	Kpn I
	hsp16.2-R	5′ CCGCTCGAGTGTCACTTTACCACTATTTCCGTCC 3′	Xho I
sod-3	sod-3-F	5′ CGGGGTACCAGCTCCTTTTAAATTAAGACA 3′	Kpn I
	sod-3-R	5′ CCGCTCGAGTATTCTTCCAGTTGGCAAT 3′	Xho I