Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 12;7:11379. doi: 10.1038/s41598-017-11563-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Lateral diffusion of IgM-BCRs and IgD-BCRs cannot be distinguished using Qdot-labelling. (A) Schematic representation of IgM- and IgD-containing BCRs. (B–F) Ex vivo splenic B cells were labelled with anti-IgM or anti-IgD (αIgD) Fab-Cy3 probes (B) or with anti-IgM or anti-IgD Fab-biotin-Qdot probes (C). In panels B and C, trajectories are plotted using a colour-coded temporal scale. The dashed lines indicate cell boundaries. Scale bars = 5 μm. Cumulative frequency plots of single-state diffusion coefficients are shown (D). Median values are indicated by the dots. In panel (E), tracks were analyzed using the FPT algorithm. Histograms depicting the confinement radii for short tracks (50 frames, left panel) and long tracks (300 frames, right panel) are shown. In panel (F), the tracks were analyzed using the two-state HMM. The transition rates (K_slow→fast, K_fast→slow) between the two states are shown. The inset shows the fraction of time that receptors exhibited slow (red) versus fast (blue) diffusion, as determined using the two-state HMM. All data in panels (B–F) are from the same experiment. Similar results were obtained in two independent experiments.