Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 12;7:11379. doi: 10.1038/s41598-017-11563-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Larger changes in receptor mobility can be detected using Qdots. (A–C) Ex vivo splenic B cells were labelled with either anti-IgM Fab-Cy3 or anti-IgM Fab-biotin-Qdot. The cells were treated with DMSO or 1 μM latrunculin A (LatA) for 5 min prior to SPT imaging. Cumulative frequency plots of single-state diffusion coefficients for IgM-BCRs are shown in (A). Median values are indicated by the dots. (B) The tracks were analyzed using the two-state HMM. The transition rates (K_slow→fast, K_fast→slow) between the two states are shown. The inset shows the fraction of time that receptors exhibited slow (black) versus fast (orange) diffusion, as determined using the two-state HMM. All data are from the same experiment. Similar results were obtained in three independent experiments. (C) The trajectories were analyzed using the FPT algorithm and histograms depicting the confinement radii for short tracks (50 frames, left panel) and long tracks (300 frames, right panel) are shown. (D–F) Splenic B cells were cultured for 16 h with 5 ng/mL BAFF or with BAFF + 5 μg/mL LPS before being labelled with either anti-IgM Fab-Cy3 or anti-IgM Fab-biotin-Qdot. (D) Cumulative frequency plots of diffusion coefficients are shown. (E) The transition rates between slow- and fast-diffusion states determined by the HMM algorithm. The fraction of time that receptors exhibited slow (black) versus fast (orange) diffusion are shown in the inset. (F) The trajectories were analyzed using the FPT algorithm and histograms depicting the confinement radii for short and long tracks are shown. All data are from the same experiment. Similar results were obtained in two independent experiments.