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. 2017 Sep 12;7:11406. doi: 10.1038/s41598-017-11784-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microwave irradiation induces mitochondrial damage without a heat-stress response. After microwave irradiation or thermal treatment followed by a 3-h incubation period, cells were examined for (A) Bcl-2 and Bax expression; (B) mitochondrial membrane potential, by staining with JC-1; and (C) HSP70 expression, measured using ELISA. The uncropped western blot images are shown in Fig. S3. Data are expressed as the means ± SD of 4 independent experiments. *P < 0.05 and **P < 0.01 versus the negative control.