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. 2017 Sep 12;7:11268. doi: 10.1038/s41598-017-11594-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exogenous expression of scFvs does not perturb memory acquisition. Flies were trained at day 5 post-eclosion using olfactory classical conditioning and tested immediately after training. Memory performance index is shown for control flies (UAS-LacZ/+; ok107-Gal4/+), flies expressing scFv9 alone (UAS-scFv9/+; ok107-Gal4/+), flies expressing scFv42.2 alone (UAS-scFv42.2/+; ok107-Gal4/+), and flies co-expressing scFv9 and scFv42.2 (UAS-scFv9/+; UAS-scFv42.2/+; ok107-Gal4/+). Five-day-old flies expressing scFv9, scFv42.2, or both perform at equivalent levels than control flies (p = 0.9999, p = 0.9989, and p = 0.9884, respectively). Error bars indicate SEM; n = 10 per group; n.s. (not significant).