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. 2017 Sep 12;7:11414. doi: 10.1038/s41598-017-11597-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) MDR Staphylococcal strains discriminated by universally conserved thermonuclease (nuc) gene. Isolated 160 Staphylococcal spp., were screened to identify at species level by PCR using universally conserved thermonuclease (nuc) gene. PCR confirmed as 151 methicillin resistant S. aureus (Lane 1–4, 359 bp), 6 as S. epidermidis (Lane 5–8, 251 bp) and 2 as S. haemolyticus (Lane 9 and 10, 434 bp) used along with E. coli (Lane 11), S. typhimurium (Lane 12), S. flexneri (Lane 13), V. cholera (Lane 14), and V. parahaemolyticus (Lane 15) were compared with 100 bp ladder (M) and water as negative control (C-). (B) Illustration of PCR amplicons expected when using the updated Staphylococcal spp. multiplex PCR. Lane M, 100 bp ladder; Lane 1, negative control (C-); Lane 2, mixture of multiplex PCR products obtained for all the templates of MDR-MRSA, MRSE, MRSH and S. typhimurium; Lane 3, MRSA; Lane 4, MRSE; Lane 5, MRSH and Lane 6, S. typhimurium comparable to the amplified product of mPCR in lane 2.