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. 2017 Sep 12;7:11414. doi: 10.1038/s41598-017-11597-z

Table 2.

Different primers used in the present study.

Sl. No. Gene/region Name Primer designation 5′→3′ Annealing temperature (°C) Molecular weight in bp Reference
1 16 S rRNA 16 S rRNA F TGGTAGTCCACGCCCTAAAC 56 210 [55]
16 S rRNA R CTGGAAAGTTCCGTGGATGT
2 nuc Saur F TCGCTTGCTATGATTGTGG 58 359 [20]
Saur R GCCAATGTTCTACCATAGC
3 Sepi F TTGTAAACCATTCTGGACCG 58 251
Sepi R ATGCGTGAGATACTTCTTCG
4 Shae F TAGTGGTAGGCGTATTAGCC 58 434
Shae R ACGATATTTGCCATTCGGTG
5 Eap Eap F TACTAACGAAGCATCTGCC 62 230 [60]
Eap R TTAAATCGATATCACTAATACCTC
6 Coa Coa F AAGATGGCACAGTATCATATGG 58 230 This study
Coa R GCCATATGTCGCAGTACC
7 SpA SpA F AGCACCAAAAGAGGAAGACAAC 58 304 This study
SpA R ATGTACTCCGTTGCCGTCTT
8 mecA mecA F TCCAGATTACAACTTCACCAGG 53 162 [50]
mecA R CCACTTCATATCTTGTAACG
9 PVL luk-PV-1 F ATCATTAGGTAAAATGTCTGGACATGATCCA 58 433 [52]
luk-PV-2 R GCATCAASTGTATTGGATAGCAAAAGC
10 Stx stx F ACACTGGATGATCTCAGTGG 57 614 [57]
stx R CTGAATCCCCCTCCATTATG
11 ompW ompW F CACCAAGAAGGTGACTTTATTGTG 59 588 [56]
ompW R GAACTTATAACCACCCGCG
12 Scaffolding protein Sf F TCTCCGCGACAGAAATCACT 56 204 This study
Sf R CCTGAACTGGACCCACTCAT
13 phoP phoP F ATGCAAAGCCCGACCATGACG 56 299 [59]
PhoP R GTATCGACCACCACGATGGTT
14 Emetic toxin Bc F GACAAGAGAAATTTCTACGAGCAAGTACAAT 58 635 [61]
Bc R GCAGCCTTCCAATTACTCCTTCTGCCACAGT
15 toxR toxR F GTCTTCTGACGCAATCGTTG 56 368 [58]
toxR R ATACGAGTGGTTGCTGTCATG