Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 12;7:11402. doi: 10.1038/s41598-017-11035-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

SmMIP-based targeted RNAseq reveals decreased expression levels in SKRC7-VHL cells of glycolysis related genes a.o. SLC2A1, CA9, HK2 and LDHA in two independent duplicate experiments (a,b). Relative values were comparable to those obtained from whole transcriptome RNAseq analysis (c), which is in agreement with the correlation shown in Fig. 3. Differences in expression levels were validated on the protein level for HK2 and CA9, using tubulin as house keeping control (Fig. 4d). Gene transcript levels of hypoxia inducible genes are very high in surgically obtained clear cell renal cell cancer samples, relative to peritumorally obtained normal kidney tissue (e, *p = 0.01; **p < 0.003, ***p < 0.0003, Students’ T-test) (Fig. 4e).