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. 2017 Sep 12;7:11247. doi: 10.1038/s41598-017-10982-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Heparin competition assay of PgP/pDNA polyplexes (2 µg pDNA). (A) PgP/pDNA polyplexes at varying N/P ratios and bPEI/pDNA polyplex at N/P ratio 5/1 were prepared and incubated in the presence of heparin (heparin/polyplexes ratio of 3/1 (w/w)) at 37 °C for 30 min. (B) PgP/pDNA polyplexes (2 µg pDNA) at N/P ratio of 30/1 incubated with solutions containing heparin at varying concentration (0–14 heparin/pDNA (w/w) ratios) at 37 °C for 30 min. M: Molecular marker, N: naked DNA.