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. 2017 Sep 12;7:11247. doi: 10.1038/s41598-017-10982-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Long-term stability of PgP/pDNA polyplexes (N/P ratio of 30/1, 2 µg pDNA) in water at 4 °C. (A) Gel retardation assay of PgP/pDNA polyplexes stored at 4 °C for different time periods. Molecular weight marker (M, Lane 1), naked pDNA (N, lane 2), PgP only (lane 3), PgP/pDNA polyplexes at various time points during storage at 4 °C for 0, 6hrs, 1, 3, and 7 days, and 1, 3, 4, 5, and 6 months (lane 4–13). (B) Transfection efficacy of PgP/pGFP polyplexes stored at 4 °C in B35 cells in media containing 10% serum. *p < 0.05 compared with freshly prepared polyplexes. (C) Representative images and (D) Flow cytometry histogram of GFP + B35 cells at 2 days post-transfection with PgP/pGFP polyplexes stored at 4 °C. Scale bar indicates 200 µm. (Fresh: freshly prepared polyplexes, W: week, and M: Month).