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. 2017 Sep 12;7:11247. doi: 10.1038/s41598-017-10982-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability and transfection efficiency of PgP/pGFP polyplexes after lyophilization. (A) Stability of PgP/pGFP polyplexes (N/P ratio 30/1) freshly prepared and after lyophilization alone or with the inclusion of cryoprotectants. Molecular weight maker (Lane 1), naked plasmid GFP (lane 2), fresh polyplexes (lane 3), reconstituted polyplexes after lyophilization (lane 4), fresh polyplexes with 5% glucose (lane 5), reconstituted polyplexes after lyophilization with 5% glucose (lane 6), fresh polyplexes with 0.9% NaCl (lane 7), reconstituted polyplexes after lyophilization with 0.9% NaCl (lane 8). (B) Transfection efficacy of freshly prepared and lyophilized PgP/pGFP polyplexes in B35 cells. *p < 0.05 compared with freshly prepared polyplexes. ^# p < 0.05 compared with lyophilized polyplexes without cryoprotectant. (C) Representative images and (D) flow cytometry histogram of GFP+ B35 cells at 2 days after transfection with PgP/pGFP polyplexes. Fresh: Freshly prepared polyplexes, Lyo.: Lyophilized PgP/pGFP, Lyo. 5% Glucose: Lyophilized PgP/pGFP with 5% glucose, Lyo. 0.9% NaCl: Lyophilized PgP/pGFP with 0.9% NaCl. Scale bar indicates 200 µm.