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. 2017 Sep 12;7:11247. doi: 10.1038/s41598-017-10982-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Retention of DiR-PgP/pDNA polyplexes (N/P ratio of 30/1) after local injection in SCI lesion site. (A) Visualization of DiR-PgP/pβ-gal polyplexes at 1, 3, 24 hours, and 3, and 5 days following injection by live animal fluorescence imaging system (IVIS). Left: Uninjected SCI animal (Control), Right: DiR-PgP/pβ-gal polyplexes injected animal (B) Ex vivo fluorescent imaging of DiR-PgP/pDNA polyplexes at 5 days post-injection by IVIS.