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. 2017 Sep 12;7:11277. doi: 10.1038/s41598-017-11842-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OA induces cell cycle arrest in the G0/G1 phase in TSCC cells. The TSCC cells were treated with different concentrations of OA (0, 50, 100, or 150 μM) for 24 h. (a) The flow cytometric histograms show the cell cycle arresting effect of OA in CAL27 and UM1 cells. (b,c) Cell cycle arrest during the G0/G1, S, and G2/M phases was found after treatment with OA. The results are presented as the mean ± standard deviation for three independent experiments. (d,e) The G0/G1 cell cycle regulator CyclinD1 was detected by Western blot. GAPDH was used as the internal control.