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. 2017 Sep 12;7:11405. doi: 10.1038/s41598-017-11319-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distinct neuronal neurofascin gene (Nfasc) splicing across the adult brain. (a) Schematic diagram outlining partial genomic organization of Nfasc at ex21-30 and the alternative splicing patterns that generate four protein splice isoforms. Exon positions of each alternatively spliced segment (ex22-23, ex26-27, and ex28-29) are indicated by different boxes. (b) Detection of alternative splicing choices at ex26-29 (neuron-specific splices) by semi-quantitative RT-PCR. Primer sets used for the analysis were designed on the neighbouring constitutive exon sequence. Different splicing patterns at ex26-29 among brain areas was largely consistent with the pattern of protein isoforms in data shown in Fig. 1 (n = 3 animals). (c) Quantification of total Nfasc (ex24-25), Nfasc155 (ex22-23), and Nfasc186 (ex27-28) levels by RT-qPCR. Total Nfasc: F (4,14) = 3.919 (P < 0.05); Nfasc155: F (4,14) = 2.188 (P = 0.15); Nfasc186: F (4,14) = 4.581 (P < 0.05), one-way ANOVA. Asterisks show the significant differences when compared between all the tissues with post-hoc test (Bonferroni’s multiple comparison test) (n = 3 animals).