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. 2017 Sep 12;7:11405. doi: 10.1038/s41598-017-11319-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Developmental change and alternative splicing of neuronal Nfasc in the adult cerebellum. (a) Developmental profile of ex26-29 inclusion in the cerebellum and cortex. The amount of each neuronal Nfasc isoform were changed dynamically during development. (b,c) Quantification of Nfasc180 and Nfasc186 levels by semi quantitative PCR to produce the results shown in (a). (b) Nfasc186. (c) Nfasc180. Band intensity of each Nfasc isoform was normalized to that of total Nfasc (ex24-25). (d) Quantification of ex27-28 inclusion (Nfasc186) at postnatal day 0 (P0) and adulthood (2–3-months-old) in the cortex and cerebellum by qRT-PCR. (n = 3 animals) Values for cerebellum at P0 were set to 1.0.