Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 12;7:11405. doi: 10.1038/s41598-017-11319-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cell type–specific alternative splicing of Nfasc in the adult cerebellum. (a) Comparison of the inclusion patterns at ex22-23 and ex26-29 between Nfasc in whole cerebellar tissue and in granule-cell (GC) cultures. Inclusion of ex26-29 was remarkably less in GC cultures. (b) Inclusion patterns at Nfasc ex22-23 and ex26-29 between time points in developing GC cultures. Inclusion of ex26-29 did not occur developmentally in GC cultures. (c) Illustration of separation and cell isolation by laser-capture microdissection (LCM). The Purkinje cell (PC) layer (PCL), granule layer (GL), and deep cerebellar nuclei (DCN) were isolated from single cerebellar slices. (d) Representative images of LCM isolates in the cerebellar cortex and verification by standard PCR using primer sets for cell type-specific markers; Pcp2 (for PCs) and Cbln1 (for GCs). Scale bar = 100 μm. (e) RT-qPCR analysis using LCM-isolated cerebellar tissue. Nfasc186 containing ex27-28 was 4–5 fold higher in the PCL and DCN than in the GL (n = 4 animals). Values for GL were set to 1.0. Differences were compared to the values for GL. (f) A ratio of Nfasc186 in the PCL and GL by qRT-PCR analysis (n = 4 animals). Approximate percentage of Nfasc186 isoform was largely estimated from CT value of ex27-28 directly compared to that of total Nfasc (ex24-25) at the same threshold set for CT value. Mean CT values for total Nfasc were set to 100%.