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. 2017 Sep 12;7:11262. doi: 10.1038/s41598-017-11361-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Structural comparison. (a) Superimposition of GacS_PD (colored secondary structure) and two structural homologues, E. coli DcuSp (dark grey), and K. pneumonia CitAp (light grey). (b) Close-up views of the conservation pattern made by the two positively-charged residues between GacS_PD, CitAp and DcuSp. (c) Structural sequence alignment of GacS_PD, CitAp and DcuSp. The conserved Arg and His residues are highlighted with orange stars.