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. 2017 Sep 12;7:11328. doi: 10.1038/s41598-017-11102-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Soluble Htt25QP blocks endocytosis in cells lacking Myo5. (a) Images of wild type and myo5Δ cells expressing HTT25QP and HTT25Q and their capacity of endocytic uptake. Scale bar = 5 µm. (b) Quantification of a. Percentage of cells with detectable Htt levels capable of internalizing FM4-64 via the endocytic pathway. Average values from three individual experiments, with more than 150 cells per experiment, are shown. Error bars corresponds to the standard deviation, P = 0.00284. (c) Native PAGE of protein extracts from wild type and myo5Δ cells expressing HTT constructs. Representative picture from three independent experiments is shown. (d) Sucrose density gradient fractionation of protein extracts from wild type and myo5Δ cells expressing HTT25QP. The percentage of Htt25QP protein present in each fraction is quantified in graphs below each Western blot. Values are average from three independent experiments with error bars corresponding to the standard deviation. (Full-length blots are shown in Fig. S12) (e) Fluorescence micrographs of exponentially growing myo5Δ yeast cells expressing indicated HTT constructs. Scale bar = 5 µm.