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. 2017 Sep 12;7:11328. doi: 10.1038/s41598-017-11102-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Htt25QP limits type-1 myosin activity. The depolarized state and reduced endocytosis of myo5Δ Htt25QP-producing cells can be partially suppressed by overexpressing the other type-1 myosin MYO3. (a) 5x serial dilutions of wild type and myo5Δ cells expressing indicated constructs. Representative images from three individual experiments are shown. (b) MYO3 transcript levels normalized to myo5Δ + vector in cells harboring HTT25QP plasmid. Values are average from two independent experiments with error bars corresponding to the standard deviation. (c) Fluorescence micrographs of wild type and myo5Δ cells expressing different constructs used in this study and stained for F-actin structures. Maximum projection is shown. Scale bar = 5 µm. (d) Quantification of c. Graph shows percentage mother cells having more than six actin patches. Cells not expressing HTT fragments at detectable levels are excluded from the analysis. A total of ten Z-stack images were analyzed per field of view. Average values from three individual experiments using two different clones with more than 150 cells per experiment are shown. Error bars correspond to the standard deviation, P = 0.0087. (e) Fluorescence micrographs showing the cells’ capability of endocytic uptake. Scale bar = 5 µm. (f) Quantification of e. Graph shows percentage cells with endocytic vesicle(s). Cells not expressing HTT fragments at detectable levels are excluded. Average values from three individual experiments using two different clones with more than 150 cells per experiment are shown. Error bars correspond to the standard deviation, P = 0.020.