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. 2017 Sep 12;7:11333. doi: 10.1038/s41598-017-11882-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Modulating OsHsp18.0 expression influenced the accumulation of free SA and the expression of SA-related genes. (a) Free SA level in the OsHsp18.0-overexpressing plants. (b) Free SA level in the OsHsp18.0-RNAi plants. Samples from the wild type and respective transgenic lines were collected at 0, 12 and 24 hpi with Xoo strains Zhe173, PXO99 and PXO79. Bars represent means (three replicates) ±SD. Asterisks indicate that a significant difference (*p < 0.05) was detected between transgenic plants and the wild type. (c) Effect of OsHsp18.0 overexpressing on the expression of six SA-related genes. Samples were collected at 0, 12 and 24 hpi with Xoo strain Zhe173. The relative abundance of six transcripts was analyzed using qRT-PCR with primers listed in Table S1. The normalized expression level of each gene in wild-type control was set as 1. Values represent the mean ± SD from triplicate experiments.